Antifungal Activity of <i>Poncirus trifoliata</i> Roots against Colletotrichum Species

Fruits and vegetables are an essential part of a healthy diet, providing humans with vitamins, phytonutrients, minerals. They significantly vulnerable, however, to post-harvest diseases caused by numerous fungal bacterial pathogens. These pathogens can cause significant quantitative qualitative losses from harvest consumption during the handling storage processes. Chemical fungicides commonly used but likely leave residues on produce, rendering short shelf-life such as berries, unsuitable for human consumption. Identifying eco-friendly methods control disease is, therefore, utmost importance. The presence antifungal constituents in roots Poncirus trifoliata extracts was detected thin layer chromatography-based bioautography. active were isolated identified bioautography assay-guided fractionation using flash chromatography followed spectroscopic techniques. In this study, xanthoxyletin, demethylsuberosin, dentatin, nordentatin, ponfolin, clausarin root extracts. activity these compounds moderate weak compared commercial fungicide captan. This study reports isolation identification natural that exhibited against Colletotrichum fragariae Botrytis cinerea, two major